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mtecs  (ATCC)
96
ATCC mtecs
( A ) The thermal effect of LIPUS with different duty cycles on <t>MTECs.</t> ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 <t>ng/mL</t> <t>TGF-β1</t> ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Mtecs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtec culture medium  (PromoCell)
94
PromoCell mtec culture medium
( A ) The thermal effect of LIPUS with different duty cycles on <t>MTECs.</t> ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 <t>ng/mL</t> <t>TGF-β1</t> ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Mtec Culture Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtec agar  (Difco)
86
Difco mtec agar
( A ) The thermal effect of LIPUS with different duty cycles on <t>MTECs.</t> ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 <t>ng/mL</t> <t>TGF-β1</t> ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Mtec Agar, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse tubular epithelial cells mtecs  (Procell Inc)
86
Procell Inc mouse tubular epithelial cells mtecs
( A ) The thermal effect of LIPUS with different duty cycles on <t>MTECs.</t> ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 <t>ng/mL</t> <t>TGF-β1</t> ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Mouse Tubular Epithelial Cells Mtecs, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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modified mtec agar (eccfu)  (Difco)
90
Difco modified mtec agar (eccfu)
( A ) The thermal effect of LIPUS with different duty cycles on <t>MTECs.</t> ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 <t>ng/mL</t> <t>TGF-β1</t> ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Modified Mtec Agar (Eccfu), supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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modified mtec agar criteriontm  (Hardy Diagnostics)
90
Hardy Diagnostics modified mtec agar criteriontm
( A ) The thermal effect of LIPUS with different duty cycles on <t>MTECs.</t> ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 <t>ng/mL</t> <t>TGF-β1</t> ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.
Modified Mtec Agar Criteriontm, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) The thermal effect of LIPUS with different duty cycles on MTECs. ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 ng/mL TGF-β1 ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: JCI Insight

Article Title: Low-intensity pulsed ultrasound stimulation to treat renal fibrosis through inhibiting tubular IL-1R

doi: 10.1172/jci.insight.186892

Figure Lengend Snippet: ( A ) The thermal effect of LIPUS with different duty cycles on MTECs. ( B and C ) Flow cytometry of apoptosis of MTECs treated with LIPUS at varying duty cycles ( B ) and intensities ( C ) and quantification of apoptotic cells (Q2 + Q3). ( n = 3). ( D ) Protein levels of α-SMA in MTECs induced with 15 ng/mL TGF-β1 ( n = 6). ( E ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities ( n = 4). ( F ) Protein levels of α-SMA in MTECs treated with LIPUS at varying intensities stimulated by 15 ng/mL TGF-β1 ( n = 5). Data are presented as mean ± SD and were analyzed using 1-way ANOVA with Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Following serum starvation, MTECs (catalog CCL-139, ATCC) were exposed to recombinant human TGF-β1 (catalog AF-100-21C, Peprotech) at 15 ng/mL or rIL-1β at 5, 10, and 20 pg/μL.

Techniques: Flow Cytometry